sLEP-R ELISA is for the quantitative determination of human soluble leptin receptor (sLEP-R), product number R07.

The adipokine leptin realizes signal transduction via four different leptin receptor (LEP-R) isoforms. However, the amount of functionally active LEP-R is influenced by constitutive cleavage of the extracellular domain. The product of the cleavage process, called soluble leptin receptor (sLEP-R), is the major binding protein for leptin in human blood and modulates its bioavailability. Concentrations of sLEP-R are differentially regulated in metabolic disorders, such as type 1 diabetes mellitus or obesity, and may therefore increase or decrease leptin sensitivity. Lipotoxicity and apoptosis increase LEP-R cleavage via ADAM10-dependent mechanisms. In contrast, although increased levels of sLEP-R appear to directly inhibit leptin effects, reduced levels of sLEP-R may reflect decreased membrane expression of LEP-R. These results explain, in part, changes in leptin sensitivity that are related to changes in serum sLEP-R concentrations observed in metabolic disorders.

sLEP-R ELISASoluble Leptin Receptor ELISA
Product numberR07
regulatory status (EU)RUO
Calibration Recombinant eukaryotically expressed soluble leptin receptor.
Incubation period 5 h
Antibody Specific high-affinity antibodies
Standard 8 individual standards, 0-30 ng/mL
Assay Range
Sample matrixSerum / Plasma
Sample dilution 1:5
analyt. Sensitivity
Intra-/Interassay Variance [%] < 10%
Package insert
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