IGF-I human

Insulin-like growth factor I (IGF-I) plays a key role in the regulation of proliferation and differentiation of many cell types. The protein consists of 70 amino acids and has a molecular weight of 7.649 kDa.

The measurement of IGF-I concentration finds its clinical application in the analysis of growth disorders such as growth hormone deficiency or Acromegaly.

The IGF-I concentration in human serum is influenced by various factors, such as diet/food intake, hepatic diseases or obesity. However, the essential factor is the age of the subject. For this reason, age-specific reference values must be used to assess the test result.

Mediagnost offers you 3 different types of services based on the international standard. WHO 02/254 calibrated (conversion factor: 0.75) test systems for the measurement of human IGF-I in serum and plasma samples with corresponding reference values for the assessment of the measurement result.

An overview of the properties of the respective test systems can be found in the table below, detailed information can be found in the package insert and is available upon request.

IGF-I	ELISA	RIA	RIA-CT
Product number	E20	R20	R22
Regulatory status EU	IVD	RUO	IVD
Reference material	WHO 02/254	WHO 02/254	WHO 02/254
Incubation period	1,75 h	2 days + 1,5 h	4 h
Antibody	Mouse / Goat	Rabbit	Rabbit
Standard	rec. IGF-I	rec. IGF-I	rec. IGF-I
Assay Range [µg/L]	0,09 – 1050	0,064 – 1010	0.1 – 780
Samples	Serum EDTA / Heparin Plasma	Serum EDTA / Heparin Plasma	Serum EDTA / Heparin Plasma
Sample dilution	1:21	1:101	1:26
analyt. Sensitivity [µg/L]	0,09	0,064	<0,25
Intra-/Interassay Variance [%]	< 10	4,76 / 5,06	5,03 / 5,88
Package insert
Reference values	Blum W.F., Schwhttphttps://mediagnost.de/wp-content/uploads/2022/02/IFU-R22-IVD-D-Version-7.pdfs://mediagnost.de/wp-content/uploads/2022/02/IFU-R22-IVD-D-Version-7.pdfeizer R. Insulin-Like Growth Factors and Their Binding Proteins. In: Ranke MB (ed): Diagnostics of Endocrine Function in Children and Adolescents. Basel, Karger, 2003, pp.166-199.