sLEP-R ELISA Soluble Leptin Receptor ELISA
The adipokine leptin realizes signal transduction via four different leptin receptor (LEP-R) isoforms. The amount of functionally active LEP-R, however, is affected by constitutive shedding of the extracellular domain. The product of the cleavage process, the so-called soluble leptin receptor (sLEP-R, soluble Leptin receptor), is the main binding protein for leptin in human blood and modulates its bioavailability. Concentrations of sLEP-R are differentially regulated in metabolic disorders, such as type 1 diabetes mellitus or obesity and can therefore enhance or reduce leptin sensitivity. Lipotoxicity and apoptosis increase LEP-R cleavage via ADAM10-dependent mechanisms. In contrast, although increased sLEP-R concentrations seem directly to inhibit leptin effects, reduced amounts of sLEP-R may reflect decreased membrane expression of LEP-R. These findings, in part, explain alterations of leptin sensitivity that are associated with changes in serum sLEP-R concentrations seen in metabolic disorders (1-5).
| sLEP-R ELISA | Soluble Leptin Receptor sLEP-R ELISA |
| Product Code | R07 |
| Regulatory Status | RUO |
| Calibration | Recombinat eukaryotic expressed soluble Leptin Receptor |
| Inkubation time | 5 h |
| Antibody | Specific high-affinity Antibodies |
| Standard | 8 Single standards, 0-30 ng/mL |
| Assay Range [µg/L] | 0.0385-150 |
| Samplematrix | Serum / Plasma |
| Sample Dilution | 1:5 |
| analyt. Sensitivity [µg/L] | 0,0385 |
| Intra-/Interassay Variation [%] | < 10% |
| Packungsbeilage |  |