sLEP-R ELISA      Soluble Leptin Receptor ELISA

The adipokine leptin realizes signal transduction via four different leptin receptor (LEP-R) isoforms. The amount of functionally active LEP-R, however, is affected by constitutive shedding of the extracellular domain. The product of the cleavage process, the so-called soluble leptin receptor (sLEP-R, soluble Leptin receptor), is the main binding protein for leptin in human blood and modulates its bioavailability. Concentrations of sLEP-R are differentially regulated in metabolic disorders, such as type 1 diabetes mellitus or obesity and can therefore enhance or reduce leptin sensitivity. Lipotoxicity and apoptosis increase LEP-R cleavage via ADAM10-dependent mechanisms. In contrast, although increased sLEP-R concentrations seem directly to inhibit leptin effects, reduced amounts of sLEP-R may reflect decreased membrane expression of LEP-R. These findings, in part, explain alterations of leptin sensitivity that are associated with changes in serum sLEP-R concentrations seen in metabolic disorders (1-5).

sLEP-R ELISASoluble Leptin Receptor sLEP-R ELISA
Product CodeR07
Regulatory StatusRUO
Calibration Recombinat eukaryotic expressed soluble Leptin Receptor
Inkubation time 5 h
Antibody Specific high-affinity Antibodies
Standard 8 Single standards, 0-30 ng/mL
Assay Range
SamplematrixSerum / Plasma
Sample Dilution 1:5
analyt. Sensitivity
Intra-/Interassay Variation [%] < 10%
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