Leptin is a hormone predominately synthesized by adipocytes. It consists of 167 amino acids and shows a molecular weight of 18,64kDa. Leptin is involved in the regulation of food intake and the Leptin concentration in human serum is mainly determined by body fat mass.

Mediagnost offers three different Leptin test systems, all are calibrated against the international standard NIBSC 97/594. Further, we offer bioLEP, a test system to measure biologically active Leptin. Here Leptin is measured by a combination of the leptin binding protein (extracellular part of the receptor) and a specific polyclonal antibody. This combination allows to measure only functional Leptin, which is able to bind to its receptor. An overview of major assay characteristics is given in the table below.  As well as  an ELISA  Kit for quantitative determination of human Soluble Leptin Receptor (sLEP-R),  Product Code R07.

ELISA Sensitiv
Product number L07 E07 E077 R44
regulatory Status (EU) RUO IVD IVD


Reference material NIBSC 97/594 NIBSC 97/594 NIBSC 97/594 NIBSC 97/594
Incubation time 4 h 1.75 h 1.75 h 15 h
Antibody Rabbit Mouse Mouse Rabbit
Calibrator recom. Leptin recom. Leptin recom. Leptin recom. Leptin
Assay Range
1 –
0.25 – 100 0.01 – 50 0.1 – 64
Sample matrix Serum/ Plasma Serum/ Plasma Serum/ Plasma

Serum/ Plasma

Sample dilution 1:20 non diluted 1:10 non diluted
analyt. Sensitivity
0.01 < 0.25 0.01 0.1
Intra-/Interassay Variance [%] < 10 < 15 < 10 < 10
Reference values Blum WF, Juul A; Reference ranges of serum leptin, In: Leptin- the voice of adipose tissue, Blum WF et al. eds. Johann Ambrosius Verlag, Heidelberg, 1997

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