Enzyme immunoassay for the qualitative and semi-quantitative detection of human IgG antibodies against the extracellular proteins alkaline protease, elastase and exotoxin A of Pseudomonas aeruginosa.

The measurement of these antibodies in addition to classical microbiology allows a more sensitive detection of pseudomonas infection. The diagnostic value of this test system was evaluated and published by Kappler et al and Tramper-Stranders et al. A general review regarding serology in the detection of pseudomonas aeruginosa infections was published by Mauch und Levy.

 

anti-Pseudomonas aeruginosa IgG ELISA

Product Number

E15

Regulatory Status in the EU

IVD

Reference material

not available

Incubation time

4,5h

Pseudomonas Antigen

alkaline Protease
Elastase
Exotoxin A

Standard

positive humane serum

Assay Range
[Titer/mL]

0,2 – 2500

Sample matrix

Serum / EDTA-Plasma

Sample dilution

1:100 /1:1000/1:10000

analyt. sensitivity
[Titre/mL]

<0,2

Intra-/Inter assay variance [%]

<20%
at titers of > 1250

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